Schematic diagram of the four groups. (IMAGE)
Caption
Tlr4fl/fl mice were crossbred with Tlr4ΔIEC mice, and the female mice were intraperitoneally injected with 50 μg/kg of lipopolysaccharide (LPS) or an equivalent volume of phosphate buffer saline (PBS) at gestational day 14.5. After genotyping, the offspring mice were divided into four groups: Tlr4fl/fl-PBS, Tlr4fl/fl-LPS, Tlr4ΔIEC-PBS, and Tlr4ΔIEC-LPS. Intestinal motility and fecal water content were measured at 6 weeks of age. Colon tissue was collected at 8 weeks of age.
Credit
Ruifang Luo, Yuan Miao, Riqiang Hu, Fang Lin, Junyan Yan, Ting Yang, Lu Xiao, Zhujun Sun, Yuting Wang, Jie Chen
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CC BY-NC-ND