Morphological observation and intestinal motility changes in the colon of offspring mice. (IMAGE)
Caption
(A) The hematoxylin and eosin staining of colon samples from the offspring of Tlr4fl/fl and Tlr4ΔIEC mice treated with phosphate buffer saline (PBS) or lipopolysaccharide (LPS) provided an overview of the colon in both groups. Scale bar = 250 μm (n = 3 per group). (B) Histogram of colonic tissue mucosal layer thickness quantification in offspring mice of the four groups. (C) The colonic transit time in the offspring of Tlr4fl/fl and Tlr4ΔIEC mice after treatment with LPS or PBS (n = 10 per group; two-way ANOVA). (D) Fecal water content in the Tlr4fl/fl and Tlr4ΔIEC offspring with or without LPS exposure (n = 10 per group; two-way ANOVA). (E) The gastrointestinal transit time of the offspring mice in the Tlr4fl/fl and Tlr4ΔIEC groups with or without LPS treatments (n = 10 per group; two-way ANOVA). In the combined Tlr4fl/fl and Tlr4ΔIEC groups, as well as in the combined PBS and LPS groups (n = 20 per group), Tlr4 knockout or LPS treatment independently affected gastrointestinal transit time. Data were presented as mean ± standard error of the mean. The term “Interaction” denotes the effect of LPS on the Tlr4ΔIEC mice compared with Tlr4fl/fl mice. ns, not significant; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗∗P < 0.0001, and ∗∗∗∗P < 0.0001.
Credit
Ruifang Luo, Yuan Miao, Riqiang Hu, Fang Lin, Junyan Yan, Ting Yang, Lu Xiao, Zhujun Sun, Yuting Wang, Jie Chen
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.
License
CC BY-NC-ND