ATF6 function and target gene pathway analysis. (IMAGE)
Caption
(A) ATF6 binding schematic diagram, based on assays for ATAC-seq and using ATF6 motif for target gene prediction (BioRender: https://app.biorender.com). (B) Functional enrichment analysis for ATF6 target genes through gene ontology (GO) and Reactome databases. (C) STAT3 was the only gene located within 500 bp of the ATF6 promoter region. ATF6 promoter region TSS 500 gene overlays eye development pathways. (D) The protein level and quantification of STAT3 and p-STAT3 in primary lens epithelial cells treated with DMSO or Ceapin-A7 (n = 3/group; mean ± standard deviation; **P < 0.01; unpaired student's t-test). (E) The protein level and quantitative chart of STAT3 and p-STAT3 in TGF-β1 mediated primary lens epithelial cells treated with DMSO or Ceapin-A7 (n = 3/group; mean ± standard deviation; **P < 0.01; unpaired student's t-test). ATF6, activating transcription factor 6; TGF-β1, transforming growth factor beta 1; STAT3, signal transducer and activator of transcription 3; p-STAT3, phosphorylated STAT3.
Credit
Huiping Lu, Xianyang Liu, Qian Zhou, Ruonan Li, Hangjia Zuo, Ke Hu, Meng Tian, Hong Wang, JianqiaoLi, Na Li, Shengping Hou
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