CREB3L1+ CB cells displayed increased interactions with fibroblasts in metastasis. (IMAGE)
Caption
(A) Single-cell identification of cells with bulk sample phenotype correlation analysis using Scissor. The t-SNE visualization of the Scissor selected cells in CB cells. The red and blue dots are Scissor+ (worse prognosis) and Scissor− (favorable prognosis) cells, respectively. (B) The bar chart showing the ratio of the number of Scissor+ cells to the number of Scissor− cells. A log2 ratio> 0 meant a positive relation with worse survival. (C) The ranked differential cross-talk between CREB3L1+ CB cells and non-malignant cells (metastatic samples versus primary samples) was indicated. Dot sizes represented the number of ligand–receptor pairs, which met the following requirements: P < 0.05 in metastatic samples and P > 0.05 in primary samples. The pink circle represented GTSE1+ OB cells, while the blue circle represented non-malignant cells. (D, E) Complement scores (D) and inflammatory response scores (E) among CREB3L1+ CB cells, FGFBP2+ CB cells, and ATAD3A+ CB cells. (F, G) Pseudotime trajectories of cancer cells generated using Monocle3. (F) The UMAP visualization of cancer cells. (G) Cells were color-coded for their corresponding pseudotime. (H) A heatmap of differential ligand–receptor cross-talk between GTSE1+ OB cells and CREB3L1+ CB cells (metastatic samples versus primary samples). The red color represented the expression value in cells. The blue color represented interaction scores, whereas the dark blue color indicated greater predicted interactions. Ligand–receptor pairs were listed along the left axis. P values were calculated by a two-tailed Wilcoxon rank-sum test. ***P <0.001.
Credit
Linzhu Wang, Wenyue Li, Weihang Ji, Danyang Bing, Mingyue Liu, Kaidong Liu, Bo Chen, Zhangxiang Zhao, Yunyan Gu, Xuelian Li, Xiaoqiang E, Lei Yang
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