Increased miR-34a induced hepatocyte ballooning degeneration and fibrogenic status. (IMAGE)
Caption
AML12 cells were transfected with Scram (control) or miR-34a mimics for 48 h and subjected to the following analysis (A–D). (A) Hematoxylin-eosin staining and microscope imaging of the transfected AML12 cells. The arrows mark the ballooned AML12 cells. Scale bar, 20 μm. (B) mRNA expression of Shh by qRT-PCR. (C) qRT-PCR analysis of Tgfβ1 and Tgfβ2 mRNAs. (D) Immunoblotting analysis of TGFβ1 and TGFβ2 and loading control β-actin proteins. (E) mRNA expression of miR-34a by qRT-PCR in AML12 cells treated with Veh (H2O) or Tgfβ2 (2 mg/mL) for 48 h. For cell treatment, two independent experiments were performed in triplicate. Results represent mean ± standard deviation. The two-tailed student's t-test was used for statistical analyses of two-group comparisons. *P < 0.05 and **P < 0.01 versus controls. qRT-PCR, real-time quantitative reverse transcription PCR; Shh, sonic hedgehog; TGF-β1/2, transforming growth factor-beta 1/2; Veh, vehicle; Scram, scramble miRNA.
Credit
Qihua Duan, Ruixiang Hu, Yan Chen, Henry Wade, Szczepan Kaluzny, Bingrui Zhang, Rongxue Wu, Guangnan Liu, Cunchuan Wang, Edward N. Harris, Qiaozhu Su
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