The CCDC86-ATF3 regulates glycolytic metabolism and tumor development. (IMAGE)

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The CCDC86-ATF3 regulates glycolytic metabolism and tumor development.

Caption

(A) Impact of CCDC86 knockdown on glycolysis-related protein levels in SHG-44 and U251 cells. (B) Effects of CCDC86 knockdown on ATP content, glucose uptake, and lactate production in SHG-44 and U251 cells. (C) Influences of the ERK pathway on glycolysis-associated protein expression in CCDC86-overexpressing cells. (D) Effects of ERK inhibitor on ATP content, glucose uptake, and lactate production in CCDC86-overexpressing cells. (E) Assessment of LDHA, PKM2, and ALDOA levels in SHG-44 and U251 cells with ATF3 knockdown, CCDC86 overexpression, and combined CCDC86 overexpression and ATF3 knockdown. (F) Assessment of ATP content and glucose uptake in SHG-44 and U251 cells with ATF3 knockdown, CCDC86 overexpression, and combined CCDC86 overexpression and ATF3 knockdown. (G) Assessment of glycolysis inhibition with 2-DG treatment in CCDC86-overexpressing cells. (H, I) Evaluation of cell proliferation (H) and apoptosis (I) following treatment with 2-DG in CCDC86-overexpressing cells. (J) Detection of ERK pathway-associated and glycolysis-related protein expression in tumor tissues with ATF3 knockdown, CCDC86 overexpression, and combined CCDC86 overexpression and ATF3 knockdown. (K) Patterns of CCDC86, ATF3, ERK pathway-associated, and glycolysis-related proteins in cancer tissues with high CCDC86 expression compared with adjacent normal tissues with low CCDC86 expression. NC (OE+KD): control; CCDC86+NC-shATF3: CCDC86 overexpression; shATF3+NC-CCDC86: ATF3 down-regulation; CCDC86+shATF3: CCDC86 overexpression and ATF3 down-regulation. *P < 0.05, **P < 0.01, and ***P < 0.001. CCDC86, coiled-coil domain-containing 86; ATF3, activating transcription factor 3; ERK, extracellular signal-regulated kinase; LDHA, lactate dehydrogenase A; PKM2, pyruvate kinase M2; ALDOA, aldolase, fructose-bisphosphate A.

Credit

Jinping Liu, Dingyu Du, Yukai Huang, Jie Tian, Xuhui Wang, Longyi Chen, Feng Wang

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