Let-7a-5p suppresses the Warburg effect in oral squamous cell carcinoma cells. (IMAGE)
Caption
(A, B) Expression levels (A) and fold changes (B) of the top 10 differential miRNA expression by exosomal ADAMTS9-AS2 in CAL27 cells. (C) Relative let-7a-5p expression in samples of patients at different stages of the disease. (D) Quantitative reverse transcription PCR was used to analyze ADAMTS9-AS2 and let-7a-5p levels after ADAMTS9-AS2 knockdown in HOK cells. (E) Glucose uptake, lactate production, ATP levels, and O2 consumption were measured in CAL27 cells transfected with non-targeting control (NTC) or let-7a-5p mimics. (F) Quantitative reverse transcription PCR analysis of let-7a expression in CAL27 cells transfected with NTC or let-7a-5p antagomirs. Glucose uptake, lactate production, and ATP levels were measured in CAL27 cells transfected with NTC or let-7a-5p antagomirs. The data were presented as mean ± standard deviation of three independent experiments by student's t-test. *P < 0.05, **P < 0.01, ***P < 0.001. A9-AS2, ADAMTS9-AS2; Exos, exosomes.
Credit
Shanghui Zhou, Jingyu Zhan, Jia Wang, Jingang Yang, Dahe Zhang, Zhenming Li, Yue He
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