Identification of major trophoblast subtypes at the maternal–fetal interface and differentially expressed genes (DEGs) via the deep learning method called deep embedding for single-cell clustering (DESC) method. (IMAGE)
Caption
(A) t-distribution random neighborhood embedding (t-SNE) plots from human placental single-cell RNA sequencing data showing the various trophoblast lineages and the trophoblastic ectoderm (TE). (B) The number of subtypes of each trophoblast lineage. (C) Annotation of the DESC method and the expression distribution of known marker genes for each lineage or cell type (HLA-G, EVT marker; CYP19A1, STB marker; CDH1, villous CTB marker; CDX2, TE marker). (D) The Venn diagram illustrating the genes that are expressed in each of the five EVT subtypes in the gene set maps. (E) Biaxial scatter plots of WIPF1 expression patterns in different trophoblast lineages and TE. (F) The violin plots showing the expression of WIPF1 in the four major trophoblast lineages. CTB, cytotrophoblast; EVT, extravillous trophoblast; STB, syncytiotrophoblast; HLA-G, human leukocyte antigen-G; CYP19A1, cytochrome P450 family 19 subfamily A member 1; CDH1, cadherin 1; CDX2, caudal type homeobox 2; WIPF1, WAS/WASL interacting protein family member 1.
Credit
Cong Li, Shengya Wang, Jing Tang, Xin Luo, Luxing Ge, Youlong Xie, Lijuan Fu, Lingling Ruan, Enoch Appiah Adu-Gyamfi, Fangfang Li, Yingxiong Wang, Hongbo Qi, Yubin Ding
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