Macrophage-specific κ-OR deficiency aggravated chronic hypoxia-induced pulmonary hypertension (HPH). (IMAGE)
Caption
(A) Flow chart of the animal experiments. After 5 days of tamoxifen administration, κ-ORfl/fl and κ-ORΔMac mice were exposed to indoor air (normoxia) or 10% oxygen (hypoxia) plus weekly subcutaneous injection of SU5416 (10 mg/kg) for 4 weeks. (B, C) Mouse peritoneal macrophages were isolated 4 weeks after HPH, and κ-OR protein expression was analyzed by western blotting (n = 3 animals). (D–F) Echocardiography revealed changes in right ventricular wall thickness (RVWT) and tricuspid ring systolic displacement (TAPSE) values (n = 10 animals/group). (G, H) Changes in right ventricular systolic pressure were determined using a micro-pressure sensor (n = 9 animals/group). (I, J) Changes in the right heart hypertrophy index (n = 3 slices). *P <0.05 and **P <0.01. κ-ORfl/fl, mice with wild-type κ-OR in myeloid cells. κ-ORΔMac, mice with myeloid-specific deletion of κ-OR.
Credit
Qiaojuan Wang, Jiayuan Liu, Renqi Li, Sihan Kong, Yinjie Wang, Guoyang Huang, Shumiao Zhang, Na Feng, Xiaoming Gu, Yali Liu, Ming Jia, Feng Fu, Jun Li, Juan Li, Jianming Pei
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.
License
CC BY-NC-ND