κ-OR activation exerted an anti-inflammatory effect on macrophages via the up-regulation of SCD1. (IMAGE)
Caption
(A) Scd1, Scd2, Acot1, Acsl3, Fads2, Acat2, Elovl6, Cyp51, Msmo1, Sqle, Dhcr24, Fdft1, and Tm7sf2 mRNA expression in mouse peritoneal macrophages (n = 3). (B–D) Western blotting and quantification of κ-OR and SCD1 protein expression in mouse peritoneal macrophages (n = 3). (E, F) Western blotting and quantification of SCD1 in mouse peritoneal macrophages (n = 3). (G–J) Western blotting and quantification of NLRP3, IL-1β, and IL-6 in macrophages (n = 3). (K, L) Western blotting and quantification of SCD1 in RAW264.7 cells 48 h after infection with a lentivirus harboring SCD1 (SCD1-OE) (n = 3). (M–P) mRNA expression of SCD1, IL-1β, IL-6, and TNF-α in RAW264.7 cells treated with LPS (n = 3). (Q–T) Western blotting and quantification of NLRP3, IL-1β, and IL-6 in lung tissue (n = 3). *P <0.05 and **P <0.01. LPS, lipopolysaccharide, 100 ng/mL, 24 h; AD-EGFP, adenovirus carrying EGFP, 48 h; AD-Cre, adenovirus carrying EGFP and Cre recombinase, 48 h. CAY, CAY10566, 7.5 μM.
Credit
Qiaojuan Wang, Jiayuan Liu, Renqi Li, Sihan Kong, Yinjie Wang, Guoyang Huang, Shumiao Zhang, Na Feng, Xiaoming Gu, Yali Liu, Ming Jia, Feng Fu, Jun Li, Juan Li, Jianming Pei
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.
License
CC BY-NC-ND