Wnt-C59 inhibits tumorgenicity of glioblastoma. (IMAGE)
Caption
(A, B) GSC11 (A) and U87MG (B) cells were treated with DMSO (control) or Wnt-C59 (1 μM) and growth was assessed with soft agar colony formation assay. Representative images are shown with and without Wnt-C59 treatment. The bar charts display the mean of the colony numbers of triplicate plates from a representative experiment (performed twice); Bars, standard error of the mean. ***P < 0.001 as determined using Student's t-test. (C, D) GSC11 (C) and U87MG (D) cells treated with serial doses of Wnt-C59 or control were subjected to transwell invasion assays. The data were presented as mean ± standard error of the mean. *P < 0.05, **P < 0.01, and ***P < 0.001. (E) Representative images of neurospheres of GSC11 cells after Wnt-C59 treatment. (F) The neurosphere formation efficiency was assessed in GSC11 cells. The formation assay was performed by plating dissociated single cells at a density of 1 cell/μL and counting the number of spheres that formed after 7 days. The values were mean ± standard deviation for triplicate samples. *P < 0.05 and ***P < 0.001.
Credit
Xiaowei Zhang, Zhongyong Wang, Taohui Ouyang, Brayden Wang, Richard I. Joh, Suyun Huang
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.
License
CC BY-NC-ND