Workflow of single-cell RNA sequencing experiments (IMAGE)
Caption
Initially, cells are dissociated from liver tissues into single-cell suspensions using in vivo enzymatic perfusion or ex vivo digestion methods. The cells of interest are then enriched from the suspensions through FACS or MACS. Cells are then processed using either high-throughput droplet-based microfluidic systems or microwell-based systems, followed by single-cell RNA lysis, reverse transcription, cDNA library construction and sequencing. The raw sequencing data are preprocessed through bioinformatic pipelines to generate count metrics, which are then used for computational analyses such as clustering, annotation, trajectory inference, regulatory network analysis or cell-cell communication analysis. Created with BioRender. BAM, binary alignment map; FACS, fluorescence-activated cell sorting; MACS, magnetic-activated cell sorting.
Credit
By Yankai Wen, Cynthia Ju
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Credit must be given to the creator. Only noncommercial uses of the work are permitted.
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CC BY-NC