Novel insights gained from single-cell transcriptomic studies. (IMAGE)
Caption
In single-cell transcriptomic studies, fetal-like (Afp+Cdh17+Spp1+) hepatocytes and migratory (Anxa2+) hepatocytes predominantly accumulate at the interface between necrotic areas and undamaged tissue, facilitating wound closure following ALI. Activated HSCs following ALI display distinct functions during different stages: Acta2+Col1a1− HSCs secrete cytokines and chemokines to drive inflammation during the injury phase, whereas Col1a1+ HSCs are involved in HGF production and extracellular matrix remodelling during the repair phase. Pericentral endothelial cells serve as a primary source of WNT2 and WNT9B, which are crucial for liver homeostasis and repair, while endothelial cells across all zones produce HB-EGF in response to shear stress, promoting liver regeneration. Additionally, Trem2+ macrophages play a vital role in resolving injury by phagocytosing dead cells. Collectively, these cell types coordinate to orchestrate effective liver repair post-ALI. Created with BioRender. ALI, acute liver injury; HB-EGF, heparin-binding EGF-like growth factor; HGF, hepatocyte growth factor; HSCs, hepatic stellate cells.
Credit
By Yankai Wen, Cynthia Ju
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Credit must be given to the creator. Only noncommercial uses of the work are permitted.
License
CC BY-NC