Oxidative phosphorylation was down-regulated after tirzepatide treatment. (IMAGE)
Caption
Transcriptomic sequencing was performed on liver tissues from three groups of mice: the NCD, HFFC, and HFFC with tirzepatide treatment groups. (A) Principal component analysis. (B) Volcano plot of differential expression. (C) KEGG enrichment analysis was performed on the differentially expressed genes between the HFFC group and the HFFC with tirzepatide treatment group. (D) Cluster analysis was performed on the genes involved in the reactive oxygen species and oxidative phosphorylation pathways across the NCD, HFFC, and HFFC with tirzepatide treatment groups. (E) Quantitative PCR was performed to validate the expression of genes in this pathway, including Atp5mc2, Gsta2, Nox4, Slc26a2, Hif1α, and Cycs. (F) Western blot analysis was used to detect Cyc protein expression in liver tissues from the NCD, HFD, HFFC, and HFD or HFFC with tirzepatide treatment groups. (G) Western blot analysis was used to detect the protein expression levels of ATP5A1, UQCRC1, SDHB, MTCO2, NDUFB8, and COX4 in liver tissues from the NCD, HFD, HFFC, and HFD or HFFC with tirzepatide treatment groups. (H) OPA1 protein expression was analyzed via western blotting. n = 5. The data are presented as mean ± SD.
Credit
Yun Li, Wencong Sun, Hong Liu, Xiong Z. Ruan
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.
License
CC BY-NC-ND