Curved Sample Images (IMAGE)
Caption
The researchers used the microscope to simultaneously acquire brightfield and fluorescence images of onion skin laid over a gently curved surface (a). Whole-sample images (b) were acquired in both brightfield and fluorescence modes under single-focus (left) and multi-focus (right) configurations. When all the cameras were forced to a single focal plane (single focus), the edges of the images are blurred. But when each camera was focused on the local curvature (multi focus), the entire curved layer stayed sharp. Fluorescence (c) and brightfield (d) zoom-ins and line profile, respectively, under single-focus (left) versus multi-focus (middle).
Credit
Haitao Chen, Duke University
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