The NBL1 gene plays a crucial role in the metastasis of OC and is associated with a poor prognosis for patients. (IMAGE)
Caption
(A) Schematic design of an experiment for identifying metastasis-related genes using a CRISPR/Cas9 library in an orthotopic murine model of OC. (B) The bioluminescence signals and quantifications of CRISPR Lib and Vector group mice upon intraperitoneal injection of SK-OV-3 cells from day 8 to day 21 are shown in the images (above) and graphs (below) (n = 8; mean ± standard error of the mean, 2-way ANOVA; **P < 0.01, ***P < 0.001). (C) Differences in gene expression are depicted in the figure, with red representing the relatively higher gene expression level and blue representing the relatively lower gene expression. The horizontal dotted line above indicates genes with Padj < 0.05, while the vertical dashed lines on both sides represent genes with |log2fold-change| > 1.5. (D) The Venn diagram compares the hits that meet the specified criteria. (E, F) Real-time quantitative PCR analysis of NBL1 expression in human primary OC tissues (Pri) and paired peritoneal metastases (Met). Data were normalized to GAPDH (endogenous control) using the 2−ΔΔCt method. Values represent mean ± standard error of the mean from three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001; paired two-sided student's t-test). (G, H) Kaplan–Meier survival plot of the overall survival (OS) and progression-free survival (PFS) of serous OC patients with different NBL1 expression. (I) Correlation analysis of NBL1 expression with the clinical stage of OC patients. NBL1, neuroblastoma suppressor of tumorigenicity 1; OC, ovarian cancer.
Credit
Yue Qi, Wenwen Zhang, Xinyu Li, Yi Shi, Pengpeng Qu
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