Figure 2: Intracellular delivery in human gingival fibroblasts (hGFs) using the 2D cell‑squeezing device. (IMAGE)
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(a) 6‑FAM siRNA; (b) EGFP plasmid. For each condition, (i) bright‑field to locate cells, (ii) green fluorescence reporting intracellular cargo, (iii) Calcein red‑orange as the live/dead readout, and (iv) image‑cytometry overlays showing per‑cell segmentation and delivery/viability class. Scale bar: 20 µm.
(c) Summary bars of delivery efficiency (green‑positive fraction) and cell viability (Calcein‑positive fraction), reported as mean ± SD across fields/replicates.
(d) Single‑cell scatter plots linking signal to state: (i) 6‑FAM siRNA (N=1,980) and (ii) EGFP plasmid (N=1,184). X‑axis, green fluorescence (a.u., delivered amount proxy); Y‑axis, Calcein red‑orange (a.u., viability proxy). These panels connect raw images to cohort metrics and per‑cell quantification in one workflow.
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