VAMP727 is retrieved from the vacuolar membrane by SORTING NEXINs. (IMAGE)
Caption
Top panels: Root epidermal cells expressing Venus-VAMP727 and mRFP-VAMP721 in wild type (left) and the snx1 mutant (right). The punctate localization pattern of VAMP721, which functions in the secretory pathway, remained unchanged in the snx1 mutant. In contrast, VAMP727 accumulated at the vacuolar membrane in the snx1 background. Scale bar = 10 µm.
Bottom panels: Images acquired using the super-resolution confocal live imaging microscope (SCLIM) of mGFP-VAMP727 (yellow), SNX1-mRFP (magenta), and the vacuolar membrane marker miRFP-SYP22 (cyan). These images capture the budding of VAMP727 together with SNX1 from the vacuolar membrane. Grid size = 0.942 µm.
Credit
Ueda Lab, NIBB
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