CD8+ T cells display a lower expression of ICB checkpoints in HPV+ PSCC. (IMAGE)
Caption
CD8+ T cells display a lower expression of ICB checkpoints in HPV+ PSCC. (A–C) UMAP plot of 14 693 TIL-Ts in Fig. 1(E). Each dot represents one single cell, coloured according to 11 clusters (A), 11 patients (B), and HPV status (C). These clusters were grouped into five cell types, including CD4+ T, PreT, CD8+ T, individual T (PT6), and proliferation T cells. Individual T cells were excluded for downstream statistical proportion analysis to reduce analytical bias. (D) UMAP visualization showing the classical marker gene expression to define major cell types. (E) Relative proportion of each cluster in 10 PSCC patients from HPV− and HPV+ PSCC. (F) Multiplex immunofluorescence depicting the distribution of CD4+ and CD8+ T cells in HPV+ and HPV− PSCC. Representative images include DAPI+ nuclei for reference. Scale bar: 200 μm. (G) Quantification of CD4+ and CD8+ T cells per area between HPV+ and HPV− PSCC (n = 3). GSEA showing pathways significantly enriched in HPV− PSCC (H, I) or HPV+ PSCC (J, K). Violin plot showing the expression of cytokine (L) or immune checkpoint (M) in CD8+ T cells by HPV status. Statistical analysis was performed using a two-sided unpaired Student's t-test for (G) and a Wilcoxon rank sum test for (L) and (M). Data are presented as mean ± SEM, with significance levels indicated as follows: ns: not significant, *P < 0.05. ICB: Immune checkpoint blockade.
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Precision Clinical Medicine
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