Fig.2 Construction of the three-dimensional atlases (CUBIC Organ/Body Atlas) (IMAGE)
Caption
(A) Detection of all cells in adult mouse organs. Each panel shows a two-dimensional section acquired by light-sheet microscopy (top left), a magnified view (top right), a magnified image with detected cells highlighted in red (bottom right), and a three-dimensional reconstruction of nuclear density (bottom left).
(B) Construction of the three-dimensional organ atlases. Based on three-dimensional images reconstructed from nuclear density, anatomical regions were segmented and color-coded to generate a 3D atlas of each organ. The upper panels show whole-organ views, and the lower panels show sagittal sections.
(C) Imaging of the whole neonatal mouse. Sagittal sections and corresponding magnified views are shown. All cells in the entire body were detected.
(D) Construction of the whole-body three-dimensional atlas. Organs were segmented and color-coded in three dimensions based on reconstructed nuclear density, generating a whole-body 3D atlas.
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