Effects of dexamethasone (Dex) on bone microstructure and TIGAR expression in femur of mice. (IMAGE)
Caption
The mouse model of osteoporosis was established by intramuscular injection of Dex (2 mg/kg and 5 mg/kg) for 8 weeks. (A) Representative three-dimensional reconstructive micro-CT images of trabecular bone and cortical bone from the control group and the Dex groups. (B) Bone mineral density (BMD), n = 5 mice. (C) Trabecular bone volume (BV/TV), n = 5 mice. (D) Trabecular number (Tb.N), n = 5 mice. (E) Trabecular separation (Tb.Sp), n = 5 mice. (F) Femur morphological analysis with H&E staining (scale bar, 200 μm). (G, H) Level of C-terminal cross linked peptide of type I collagen (CTX-I) (n = 5 mice), (G) and N-terminal propeptide of type I collagen (PINP) (H) in mouse serum. (I, J) Confocal microscopy images showing the immunofluorescence staining of TIGAR expression in femur tissue and the quantification of the integrated optical density (IOD) per field. Scale bar, 100 μm; n = 3 mice. (K, L) Western blot analysis and quantification of TIGAR in femur tissue, n = 3. Data are shown as mean ± SEM. Statistical significance was assessed by one-way analysis of variance (ANOVA) with Tukey's multiple comparisons test. ∗∗∗p < 0.001,∗∗p < 0.01, ∗p < 0.05. Dex-L, Dex dose of 2 mg/kg. Dex-H, Dex dose of 5 mg/kg.
Credit
Dingmei Zhang , Feng Ding , Yizhong Wang, Jie Cheng, Jiaxing Zhu, Shiyu Liu, Xin Wang, Zheng-Hong Qin, Lili Ren
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