Protective role of TIGAR on dexamethasone (Dex)-induced bone loss. (IMAGE)
Caption
(A) Representative three-dimensional reconstructive micro-CT images of the trabecular and cortical bones from the control and Dex groups. (B) Bone mineral density (BMD). (C) Trabecular bone volume (BV/TV). (D) Trabecular number (Tb.N). (E) Trabecular separation (Tb.Sp). (F) Femur morphological analysis by H&E staining (scale bar, 200 μm). (G, H) Western blot analysis and quantification of TIGAR in femur tissue of wild type (WT) and TIGAR transgenic (TG). (I) Alkaline phosphatase (ALP) staining and quantification of ALP-positive cells showed osteoblast activity of bone marrow mesenchymal stem cells (BMSCs) derived from WT and TG mice. (J) Alizarin Red S staining and quantification of mineralized nodules showed the osteogenic differentiation ability of BMSCs derived from WT and TG mice. Data are shown as mean ± SEM. n = 3 mice. Statistical significance was assessed by two-way analysis of variance (ANOVA) with Tukey's multiple-comparisons test. ∗p < 0.05, ∗∗p < 0.01. WT, wild type; TG, TIGAR transgenic.
Credit
Dingmei Zhang , Feng Ding , Yizhong Wang, Jie Cheng, Jiaxing Zhu, Shiyu Liu, Xin Wang, Zheng-Hong Qin, Lili Ren
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.
License
CC BY-NC-ND