Event-based imaging and reconstruction of neuronal calcium dynamics (IMAGE)
Caption
Figure 2: Event-based imaging and reconstruction of neuronal calcium dynamics
a, Mean image of the hippocampal neuron culture used for calcium imaging. b, Standard-deviation projections from the reference sCMOS recording and the event-based ΔF/F₀ reconstructions. A Gaussian blur (σ = 1.5 pixels) was applied to the event data prior to s.d. computation to suppress sensor-level noise. c, Calcium traces from selected ROIs in panel b. Solid lines represent event-based reconstructions; dotted lines indicate reference sCMOS signals. Both traces were resampled at 10 Hz for comparison. d, Schematic of the awake mouse model used for in vivo cortical calcium imaging. e, Standard-deviation projection of the sCMOS signals. f, Standard-deviation projection from event-based ΔF/F₀ reconstruction, processed identically to b (with Gaussian blur applied). g, ΔF/F₀ traces from selected ROIs shown in panel f. Reconstructions were sampled at 10 Hz, and global brightness fluctuations were removed to enhance neuron-specific dynamics.
Credit
Prof. Myunghwan Choi and Prof. Kunyoo Shin at Seoul National University, Prof. Young-gyu Yoon at KAIST, Prof. Euiheon Chung at GIST, PhotoniX
Usage Restrictions
Credit must be given to the creator.
License
CC BY