In vivo cortical vascular imaging using an event camera. (IMAGE)
Caption
Figure 1: In vivo cortical vascular imaging using an event camera. a, Schematic of the mouse preparation. A mouse with a cranial window was anesthetized for cortical imaging. b, Left, widefield fluorescence image of pial vasculature acquired using an sCMOS camera. Scale bar, 20 μm. Middle, accumulated events from the event camera over time. Right, standard deviation image from naïve accumulation, highlighting three selected regions of interest (ROIs) selected for kymograph-based flow analysis. c, 100 Hz reconstructed sequential event frames displayed at 0.1s intervals. Positive (green) and negative (magenta) events correspond to RBC-induced brightness fluctuations in narrow vessels. d, Mean blood flow velocities measured from 1000 Hz reconstructions across the three ROIs. Error bars represent standard deviation over time. e, Kymographs generated from 1000 Hz reconstructions. Diagonal band slopes correspond to flow velocity. f, Mean event counts over time from a single vessel ROI (white box in left image). Alternating green and magenta spikes reflect RBC passages through the vessel.
Credit
Prof. Myunghwan Choi and Prof. Kunyoo Shin at Seoul National University, Prof. Young-gyu Yoon at KAIST, Prof. Euiheon Chung at GIST, PhotoniX
Usage Restrictions
Credit must be given to the creator.
License
CC BY