YHD combined with cisplatin (CDDP) has a synergistic inhibitory effect on the growth of OS cells (IMAGE)
Caption
(A) CCK8 assay detected the effect of CDDP on the viability of OS cells at 24 h. (B) Combined index Q of YHD and DDP. (C, D) Scratch healing assay detected the effect of the combination of CDDP and YHD on OS cell migration. (E, F) Transwell assay detected the effect of the combination of CDDP and YHD on OS cell invasion. (G, H) Flow cytometry was used to detect the effect of the combination of CDDP and YHD on apoptosis in OS cells. (I, J) Western blot analysis detected the effect of the combination of CDDP and YHD on the expression levels of related proteins in OS cells. (K) The effect of the combination of CDDP and YHD on tumorigenesis in nude mice. (L) The effect of the combination of CDDP and YHD on tumor volume and body weight in nude mice. (M) Hematoxylin-eosin staining detected the effect of the combination of CDDP and YHD on tumor tissue, lung tissue, liver, heart, and kidneys in nude mice. (N) PCNA, Bcl-2, and Vimentin of the mouse tumor tissue were detected by immunohistochemistry. Data were presented as mean ± standard deviation (n = 3 for A–J; n = 6 for K–N). ∗p < 0.05 and ∗∗p < 0.01 versus the control group.
Credit
Yanran Huang, Dagang Tang, Runhan Zhao, Jun Zhang, Xiao Qu, Ningdao Li, Yi Ren, Xiaoji Luo
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