Role of MPO and ELANE in the risk of alcohol-induced epilepsy risk (IMAGE)
Caption
(A) The PPI network illustrates the overall interactions of overlapping genes. (B) The PPI network presents the top 8 hub genes among the overlapping genes. (C) Venn diagram showing the overlapping genes identified using the LASSO, RF, SVM-RFE, and PPI methods. (D) Representative immunoblots and quantification of MPO and ELANE protein expression in mouse hippocampal lysates after acute alcohol exposure compared with those in the control group. n = 6; ∗∗∗∗p < 0.0001. (E) Representative immunoblots and quantification of MPO and ELANE protein expression in mouse hippocampal lysates after acute alcohol exposure combined with chronic KA-induced epilepsy. n = 6; ∗∗∗∗p < 0.0001. (F) Representative immunoblots and quantification of MPO and ELANE protein expression in mouse hippocampal lysates after acute alcohol exposure in the PTZ-induced seizure model group compared with those in the control group. n = 6; ∗∗∗∗p < 0.0001. (G) Schematic representation showing the experimental design. (H) Seizure latency in acute PTZ kindling model behavioral experiments. n = 7; ∗p < 0.05. (I) Survival rate in acute PTZ kindling model behavioral experiments. n = 7; ns, nonsignificant difference. (J) Kindling rate in acute PTZ kindling model behavioral experiments. n = 7; ns, nonsignificant difference. (K) Proportion of mice with a Racine score of IV-V in the acute PTZ-kindling model. n = 7; ns, nonsignificant difference. Statistical significance was determined by Student's t-test (D–F, H) or the χ2 test (I–K). Abbreviations: LASSO, least absolute shrinkage and selection operator; MPO, myeloperoxidase; RF, random forest; SVM-RFE, support vector machine-recursive feature elimination; PPI, protein–protein interaction.
Credit
Ningning Zhang, Sirui Chen, Jialing Jiang, Hong Jiang, Qing Wang, Srikrishnan Raju, Jackson G. Schumacher, Jiliang Lu, Yihe Lian, Yuansong Zhang, Yuanhang Xu, Lan Zhang, Yaqing Liu, Junqiang Li, Yiru Zhang, Yuxuan Wang, Yixue Gu, Tiancheng Wang, Xin Tian
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