Figure 3 | Pixel-level, wavelength-addressable phototoxicity verification. (IMAGE)
Caption
Figure 3 | Pixel-level, wavelength-addressable phototoxicity verification. The cell-death pattern shifts upon changing the excitation wavelength. Bright-field images (White LED), propidium iodide staining (PI; red fluorescence indicates membrane-compromised/dead cells), and merged overlays show that the control group (10 μM BR, 125 s) exhibits negligible death signals. Under 532 nm irradiation (75 s or 125 s), pronounced PI-positive signals emerge specifically in pixel regions resonant with 532 nm. When the excitation is switched to 562 nm (125 s), the PI-positive region correspondingly relocates to pixels matched to 562 nm, demonstrating a programmable photodynamic effect that selectively induces cell death as a function of both wavelength and spatial position.
Credit
Xing Fu et al.
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CC BY