Analytical performance of the MD ELAAA platform in detecting the SARS-CoV-2 N protein and viral cultures. (IMAGE)
Caption
(A) Schematic illustration of the modulation of the Ag shell layer thickness in core–shell AuNFs@Ag nanostructures leading to changes in the localized surface plasmon resonance (LSPR) and light scattering intensity. (B) Standard curve of the MD ELAAA method for different SARS-CoV-2 N proteins (0, 0.005, 0.01, 0.02, 0.05, 0.1, 0.5, 1, 2, and 5 ng/mL). Data were presented as mean ± standard deviation of triplicate results (n = 3). (C) Validation was performed using multiple proteins at a concentration of 1 ng/mL, including: SARS-CoV-2 receptor-binding domain (RBD), alpha-fetoprotein (AFP), interleukin-4 (IL-4), bovine serum albumin (BSA), influenza (InFlu) A and B proteins, to validate the specificity of the MD ELAAA platform. Data were presented as mean ± standard deviation of triplicate results (n = 3). The blank control: ns, not significant; ∗∗∗∗p < 0.0001. (D) Standard curve of the MD ELAAA method for SARS-CoV-2 virus cultures at different concentrations (0, 1, 2, 5, 10, 20, 50, 100, and 200 TCID50/mL). Data were presented as mean ± standard deviation of triplicate results (n = 3). (E) Standard curve of the ELAAA method for SARS-CoV-2 virus cultures at different concentrations (0, 10, 20, 50, 100, 200, 300, 500, and 1000 TCID50/mL). Data were presented as mean ± standard deviation of triplicate results (n = 3).
Credit
Shu Zhou, Yuxi Xu, Huan Liao, Hailong Ou, Dan Qi, Yatao Wu, Yunyi Liu, Juan Li, Jiaxuan Li, Bi Shi, Fei Zhu, Siran Zhang, Jason H. Huang, Erxi Wu, Xiaoxiao Hu
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