Tumoral B7x enhances bladder tumor growth in an immunocompetent host (IMAGE)
Caption
(A) Schematic illustration of the generation of B7x+ and B7– (control) sublines for BBN963, UPPL1541, and MB49 and their subcutaneous engraftment into wild-type C57BL/6 male mice. (B) Representative flow cytometry histogram demonstrated B7x protein expression on the tumor cell surface of B7x+ and B7x– sublines. (C) Tumor growth kinetics and survival curves for B7x+ and B7x– BBN963, UPPL1541, and MB49 sublines in immune-competent mice. Tumor volumes are shown as mean (left) and individual (right) values (BBN963, n = 28 tumors per group; UPPL1541 and MB49, n = 10 per group). Multiple unpaired t-tests were used (FDR q-value = 5%; Log-rank test). (D) Representative images of B7x+ and B7x– BBN963 tumors and their associated spleens (tumors, n = 10 per group; spleens, n = 5 per group). (E) Comparison of tumor and spleen weights between B7x+ and B7x– BBN963 tumor-bearing mice (tumors, n = 30 per group; spleens, n = 15 per group; two-tailed unpaired t-test). (F) Spleen-to-body weight ratio comparison and representative H&E-stained spleen sections from tumor-naïve, B7x+, and B7x– BBN963 tumor-bearing mice (magnification, 4×; n = 2 spleens per group; two-tailed unpaired t-tests.) Error bars represent the standard error of the mean. The data represent mean ± standard error of the mean. Data in (E) and (F) are displayed as violin plots, with each dot representing an individual tumor or spleen. Corresponding P values are provided. H&E, hematoxylin and eosin.
Credit
Marc C. Pulanco, Xiang Yu Zheng, Alexander Sankin, Deyou Zheng, Xingxing Zang
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License
CC BY-NC-ND