Construction of TIM-3-silenced chimeric antigen receptor (CAR). (IMAGE)
Caption
(A) HeLa cells were first transduced with lentivirus encoding TIM-3. Results of the flow cytometry assay to detect the expression of TIM-3 in the infected HeLa cells. (B) Results of Western blotting assay to detect the protein expression of TIM-3 in the infected HeLa cells. (C) HeLa-Tim-3 cells were transduced with lentiviruses encoding different TIM-3 shRNA sequences, and the expression of TIM-3 in HeLa-Tim-3 cells was tested. (D) Schematic diagram of TIM-3 silencing CAR, which consists of the signal peptide of U6 promoter, TIM-3 shRNA, EF1-α promoter, HER2-targeting CAR, and pWPXLD lentiviral vector. (E) The expression of TIM-3 in HeLa-Tim-3 cells was tested. TIM-3 silencing CAR was called CAR kdTim-3. CAR sTim-3 was used as a control.
Credit
Binglan Zhang, Fuping Zhu, Song Wang, Yong Huang, Shishi Yu, Shaorong Tian, Minmin Li, Pan Li, Qian Xue, Bingqiang Zhang
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.
License
CC BY-NC-ND