TIM-3 silencing induced unique gene expression in human HER2-specific chimeric antigen receptor-T (CAR-T) cells. (IMAGE)
Caption
(A) Volcano plots of HER2-specific CAR-T kdTim-3 cells versus CAR-T cells. Each dot represents a differentially expressed gene. The red dots indicate up-regulated differentially expressed genes (DEGs) that passed the screening threshold, green dots indicate down-regulated DEGs, and black dots indicate nonsignificant DEGs. (B) Statistically differentially expressed genes. (C) Heat map of DEGs. (D) Gene Ontology (GO) enrichment analysis of DEGs. (E) Gene set variation analysis (GSVA) of the genes related to TIM3 functions, including the PD-1 signaling pathway and macrophage activation pathway. Genes with significant changes in the PD1 signaling pathway and macrophage activation pathway were shown in (F) and (G). C, control group (CAR-T cells); P, experimental group (CAR-T kdTim-3 cells).
Credit
Binglan Zhang, Fuping Zhu, Song Wang, Yong Huang, Shishi Yu, Shaorong Tian, Minmin Li, Pan Li, Qian Xue, Bingqiang Zhang
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