Stem-like CD8 T cell abundance and localization in NT-I7-treated tumor tissues. (IMAGE)
Caption
(A) Representative dot plot and summary graphs for effector CD8 (TCF-1-TIM-3+; n = 11–14 mice per group) and stem-like CD8 (TCF-1+TIM-3-; n = 8–10 mice per group) subsets of activated CD8 T cells. (B) Representative hematoxylin and eosin (H&E) images of LLC-1 tumors in the lungs. (C) Summary plot of manual counts of immune aggregates in tumor-bearing lung tissues per mm2. Each datapoint represents the average #/mm2 per sample (n = 5 for control and 8 for NT-I7-treated samples). (D) Representative images of immunofluorescently stained tumor-bearing lungs from control and NT-I7-treated mice. (E) Representative 2D representation of immune cells in control and NT-I7-treated tumors (n = 2 per group). (F, G) Representative plot (F) and summary graph (G) of the location of stem-like CD8 T cells in NT-I7-treated tumors from E (n = 2). The Mann-Whitney test (A and C) and the paired t test (one-tail) (G). Horizontal lines represent the mean, and the error bars represent standard deviation. Imm., immune; aggr., aggregates.
Credit
Trang Dinh, Judong Lee, Sidra Islam, Namita Nanda, Dijana Bjelivuk, Dathan Andrews, Jiasen Zhang, Nikita L. Mani, Jin Zhou, Alexandra A. Wolfarth, Donghoon Choi, Rafi Ahmed, Joseph Skitzki, Deyu Fang, Weihong Guo, Zhenghe Wang, Rebecca C. Obeng
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