Single-cell RNA sequencing identifies SPP1+ macrophages in degenerating intervertebral discs (IMAGE)
Caption
(A, B) UMAP visualization of cell populations in the Operation and Sham operation groups, with SPP1+ Mac and SPP1- Mac populations highlighted. (C) Cell frequency analysis showing increased SPP1+ macrophages in the Operation group. (D) Heatmap of differentially expressed genes across cell populations. (E) UMAP feature plots showing the expression of marker genes, including Spp1, Emb1, Fn1e, Cd14, Ccr5b2, and Mmp9. (F) Gene Ontology enrichment analysis of differentially expressed genes in Operation vs Sham operation (Top 30 GO Terms). (G) GO enrichment comparing SPP1+ Mac vs SPP1- Mac populations. (H) Information flow analysis demonstrating enhanced SPP1 signaling in the Operation group. (I) Gene set enrichment analysis (GSEA) of the ribosome pathway showing significant negative enrichment (NES = –2.74, P = 0.001, FDR = 0.001). (J) Differential number of interactions heatmap across cell types. (K) Cell–cell communication network showing the interaction strength between cell populations. (L) SPP1 signaling pathway network in the Operation and Sham operation groups. (M) UMAP feature plots of SPP1 and Acan expression. (N, O) SPP1 signaling pathway network demonstrating macrophage-nucleus pulposus cell communication.
Credit
Qiuwei Li, Liang Kang, Kaida Bo, Peilin Jin, Chenhao Zhao, Renjie Zhang, Cailiang Shen
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