IRP1 is associated with ferroptosis and drug resistance in PCa cells. (IMAGE)
Caption
(A) IC50 curves of GEM in the six common PCa cell lines. (B) IC50 curves of GEM plus hemin in the six common PCa cell lines. (C) IC50 curves of GEM plus erastin in the six common PCa cell lines. (D) Volcano plot of the differentially expressed genes between ferroptosis inducer-resistant and ferroptosis inducer-sensitive cell lines. (E) The results of the intersection for differentially expressed genes and the gene set enrichment analysis (GSEA) database. (F) Patu-8988 cells were subcutaneously inoculated in nude mice and followed by injection with GEM only or GEM plus hemin. (G) Tumor growth curves of the two groups of nude mice (∗∗∗P < 0.001). (H) Heatmap of differentially expressed genes between GEM plus PBS and GEM plus hemin of xenograft tissue RNA sequencing. (I) IRP1 protein expression level of the six cell lines was measured by Western blotting. (J) IRP1 mRNA expression level of the six cell lines was measured by quantitative reverse transcription PCR. GEM, gemcitabine; PCa, pancreatic cancer; PBS, phosphate-buffered saline.
Credit
Zeru Li, Cheng Qin, Bangbo Zhao, Tianyu Li, Yutong Zhao, Lirui Huang, Haoyu Shi, Yiping Xie, Yutong Yan, Xiangyu Zhang, Weibin Wang
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