8-Cl-Ado suppresses lipid metabolism in liver cancer by regulating gene expression (IMAGE)
Caption
(A) The Venn diagram showing common differentially expressed genes (DEGs, p < 0.05) in HepG2 and Huh7 cells treated with 8-Cl-Ado for 24 h. (B) KEGG pathway enrichment analysis of common DEGs (top 10 pathways; 4 lipid metabolism-related pathways highlighted in red). (C) Effect of 8-Cl-Ado on lipid accumulation in HepG2 cells (Left: Representative images of oil red O staining; Right: Quantification of lipid droplet detection by oil red O staining). (D) Effect of 8-Cl-Ado on intracellular total cholesterol (CHO) and triglyceride (TG) levels in liver cancer cells. (E) Schematic diagram of CHO and fatty acid biosynthesis pathways. (F) The heatmap visualizing the expression patterns and significance (Log2(fold change)) of key genes involved in CHO biosynthesis and fatty acid metabolism (n = 20) in 8-Cl-Ado-treated HepG2 and Huh7 cells. (G) Effect of 8-Cl-Ado on the expression levels of key genes involved in CHO biosynthesis and fatty acid metabolism in HepG2 cells (n = 20). All experiments were performed with at least three biologically independent replicates (n ≥ 3). Data were presented as mean ± standard error of the mean. Statistical significance was determined using Student's two-tailed t-test for two-group comparisons or one-way ANOVA for multiple groups. ∗∗∗p < 0.001.
Credit
Jing Liu, Yongle Zhao, Xue Gong, Lin Yuan, Shengyong Yang, Wenwen Jian, Han Yan, Honglin Chen, Zhicheng Yang, Yiheng Sun, Tianle Gu, He Lu, Hongyun Zhao, Zeng Tu
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