Nicotinamide riboside chloride (NRC) supplement blunted the development of neuron damage in alcohol-induced mice. (IMAGE)
Caption
(A) Experimental protocol. All animals were divided into 4 groups: negative control (NC) group, NRC group, alcohol group, and alcohol plus NRC group. Alcohol gradient feeding was conducted for 4 weeks. 2 weeks after alcohol gradient feeding, mice were treated with NRC (350 mg/kg•d) for 2 weeks. (B) The weight changes of mice under 4 weeks of control/alcohol liquid feeding. (C) Serum alcohol content. (D, E) Serum IL6 and MCP1 levels. (F–K) Behavior test. The mice were subjected to an open field test and Morris water maze test, described as total distance (F), average velocity (G), times in the central (H), escape latency (I), platform crossing (J), and time in the target quadrant (K). (L) Four weeks later, the mice were sacrificed, and the hippocampus was collected. Hematoxylin-eosin staining was used to observe the alcohol-derived brain damage. Data quantification was shown in Figure 1M. Each group included three mice, with three independent replicates performed. All data were presented as mean ± standard deviation. P-values were determined using a two-tailed unpaired t-test. ns, not significant; nd, non-detected; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001.
Credit
Jinyang Wang, Jianan Wang, Wei Xie, Qi Shen, Chengbin Wang, Ruibing Li, Shixiong Deng
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.
License
CC BY-NC-ND