Nicotinamide riboside chloride (NRC) restored mitochondrial function through mitophagy. (IMAGE)
Caption
(A–C) Western blotting of mitophagy protein (Fundc1, Mito-LC3) expression treated with NRC. (D, E) The co-immunofluorescence of mitochondria and lysosomes indicated mitophagy. (F) Cell viability assays of HT22 cells treated with 5 μM MF094 (mitophagy inhibitor) for 6 h before alcohol exposure. (G–I) Effect of mitophagy inhibition on ATP content and mitochondrial permeability transition pore. Each value was expressed as mean ± standard deviation (n = 3 independent experiments). ns, no significant; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001.
Credit
Jinyang Wang, Jianan Wang, Wei Xie, Qi Shen, Chengbin Wang, Ruibing Li, Shixiong Deng
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