Effects of large hepatitis B surface protein (LHBs) on Akt/mTOR/Cyclin D1 pathway activation, cell cycle progression, and cellular proliferation. (IMAGE)
Caption
(A) Schematic diagram of the LHBs expression plasmid. HBV pre-S/S and posttranscriptional regulatory element (PRE) sequences were cloned into the pcDNA 3.1 vector. Site-directed mutagenesis was employed to construct plasmids encoding either wild-type LHBs (LHBs-WT) or N98T-mutant LHBs (LHBs-N98T) exclusively. (B) Western blotting results of critical components of the Akt/mTOR/Cyclin D1 pathway in Huh-7 cells expressing LHBs-WT or LHBs-N98T. (C) Results of cell cycle distribution following LHBs expression detected by flow cytometry (n = 4). (D) Proliferation assay results of Huh-7 cells expressing LHBs-WT or LHBs-N98T (n = 3). Detailed statistical results are provided in Table S1. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.
Credit
Huizhen Sun, Ling Mei, Shi Song, Qian Su, Ying Yan, Huimin Ji, Jie Ma, Le Chang, Lunan Wang
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