Truncating CNTD1 mutation abolished interaction with its meiosis partner PRR19 (IMAGE)
Caption
(A) The C-terminal amino acid sequence of CNTD1 between amino acids 184–305 is essential to mediate CNTD1–PRR19 interaction in mice. Sequence alignment between the human and mice showed a highly homologous region within the PRR19-interaction amino acid fragment between the human and mice. (B) Immunoprecipitation confirmed the interaction between the full-length wild-type CNTD1 and PRR19. By contrast, PRR19 and truncated CNTD1 were barely immunoprecipitated, demonstrating that the mutation affected their interaction, potentially affecting crossover formation during meiosis. (C) Schematic representation of the CNTD1 truncating mutation disrupts the CNTD1–PRR19 interaction.
Credit
Liwei Sun, Yi Chen, Keya Tong, Weiwei Liu, Bei Liu, Yifan Wang, Guoning Huang, Jingyu Li
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