Effect of IFNLR1 deficiency on EAU, and the percentage of Th1, Th17, Treg cells and CD11c+MHC class II in splenocytes from IFNLR1−/− EAU mice and WT EAU (IMAGE)
Caption
(A, B) Representative clinical slit-lamp (A) and hematoxylin and eosin (H&E) staining section (B) images of EAU mice on day 14 after immunization. Scale bar: 50 μm. (C) Clinical scores were measured from day 8 to day 14 after immunization (the P value was evaluated on day 14 after immunization, ∗P < 0.05, ∗∗P < 0.01). (D) Histological scores were assessed by H&E staining on day 14 after immunization. (E–H) Flow cytometric analysis was performed on splenic cells from the two groups using antibodies against IFN-γ, IL-17, Foxp3, MHC-II and CD11c. (E, G) Representative flow cytometry dot plots. (F, H) Histograms of CD4+IFN-γ+, CD4+IL-17+, CD25+Foxp3+ and CD11c+MHCII+ (n = 4–5 per group). Data are shown as mean ± SEM from two independent experiments. ∗P < 0.05, ∗∗P < 0.01; P value was corrected by Bonferroni. EAU, experimental autoimmune uveitis; WT, wild-type.
Credit
Wujiao Wang, Qingfeng Wang, Jinyu Cai, Guannan Su, Wanyun Zhang, Shuai Su, Yunfan Zheng, Chaokui Wang, Peizeng Yang
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