The effect of IFNLR1 deficiency on the function of blood-retinal barrier and RPE cells in vivo (IMAGE)
Caption
(A) Representative images of Evans blue staining in retinas from WT EAU mice and IFNLR1−/− EAU mice. Scale bar: 50 μm. (B) Representative Western blotting images and quantitative analysis of ZO-1, Claudin-5 and Occludin in retinas from the two groups (n = 4 per group). (C) Primary RPE cells were harvested from the two groups and cultured with LPS for 24 h, then the supernatants were collected for the detection of IL-6, IL-8, IL-1β, and MCP-1 by ELISA (n = 8 per group). (D) Primary RPE cells were co-treated with recombinant IFN-λ2 and LPS. The secreted levels of the pro-inflammatory cytokines IL-6, IL-8, IL-1β, and MCP-1 in the supernatants were measured by ELISA (n = 5 per group). ∗∗P < 0.01, ∗∗∗P < 0.001; P value was corrected by Bonferroni. EAU, experimental autoimmune uveitis; ELISA, enzyme-linked immunosorbent assay; LPS, lipopolysaccharide; RPE, retinal pigment epithelium; WT, wild-type.
Credit
Wujiao Wang, Qingfeng Wang, Jinyu Cai, Guannan Su, Wanyun Zhang, Shuai Su, Yunfan Zheng, Chaokui Wang, Peizeng Yang
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