Effects of photothrombotic stroke on corticospinal neurons and locomotion behaviors (IMAGE)
Caption
A) Schematic drawing of the experimental design (left) and a representative horizontal image (right) of the spinal cord showing the injection site (blue: DAPI; red: AAV-retro-tdTomato). (B) TTC staining of representative transverse brain sections from a mouse with a photothrombotic stroke of forelimb areas. RFA: rostral forelimb area; CFA: caudal forelimb area. (C–E) Representative spinal cord (C) and transverse brain images (D) stained for tdTomato showing the infection in the cervical spinal cord (C) and retrogradely labeled corticospinal neurons (D) in mice with sham injury (n = 8) or photothrombotic stroke (n = 8) with quantifications (E). n.s., no statistical significance; ∗∗P < 0.01; Student's t-test. (F–H) Performance of gross locomotion, single pellet reaching, and irregular ladder walking in mice with sham injury (n = 8) or photothrombotic stroke (n = 8) at different time points after stroke. ∗∗P < 0.01 and ∗P < 0.05; repeated measures of two-way ANOVA followed by the Bonferroni correction. Scale bars: 1 mm.
Credit
Bin Sun, Li Sun, Lixiang Zhang, Xinyu Xue, Qiuyan Tian, Lei Wu, Mei Li, Jian Huang, Hong Ni, Lixiao Xu, Chenxi Feng, Jing Ren, Hongliang Huo, Xia Zhang, Xing Feng, Wenhao Zhou, Wanliang Guo, Yaobo Liu, Rong Ju, Zhenlang Lin, Xiaofeng Yang, Xin Ding
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