Characterization of lipid droplet-associated genes (LDAGs) in hepatocellular carcinoma (HCC) and identification of LDAG-associated HCC subclasses. (IMAGE)
Caption
(A) Clustering of LD-associated proteins based on their biological roles. Proteins are grouped into functional modules based on Gene Ontology (GO) analysis and UniProt functional annotations. The solid lines represent physical interactions, as annotated in the BioGRID database. The intensity of the blue color in each node corresponds to the confidence score for the interaction. The nodes outlined in red represent proteins for which LD localization has been experimentally validated, whereas the nodes outlined in black represent proteins for which LD localization has not been validated. (B) Heatmap displaying the expression profiles of the 122 LDAGs across several HCC datasets. The color intensity represents the differential expression of each gene in the HCC and control samples within the dataset. (C) Consensus matrix heatmap based on the gene expression data of 122 LDAGs in the TCGA-LIHC cohort. The color coding represents the three distinct HCC subclasses: Cluster 1 (C1), Cluster 2 (C2), and Cluster 3 (C3). (D) Heatmap showing the expression patterns of LDAGs across the three identified HCC subgroups. (E) Clinical characteristics of the LDAG-associated HCC subgroups in the TCGA-LIHC cohort. (F) Kaplan–Meier survival analysis of the three subgroups in the TCGA-LIHC dataset. (G) Comparisons of the LDAG subtypes with eight previously reported classifications based on the TCGA data set. Statistical significance is indicated as “ns” (no significant difference); ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001.
Credit
Huiying Gu, Qiumin Wu, Haibei Zhao, JingLong Du, Zhenzhen Zhang, Juan Chen
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CC BY-NC-ND