PLIN3 knockdown inhibits hepatocellular carcinoma cell proliferation and migration. (IMAGE)

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PLIN3 knockdown inhibits hepatocellular carcinoma cell proliferation and migration.

Caption

(A, B) Quantitative PCR and Western blotting analyses of PLIN3 mRNA and protein expression in PLIN3-knockdown Huh7 and PLC/PRF/5 cells, respectively. (C) BODIPY staining was used to assess LD accumulation in PLIN3-knockdown Huh7 and PLC/PRF/5 cells. (D) Measurement of TG content in PLIN3-knockdown Huh7 and PLC/PRF/5 cells. (E) Cell proliferation was assessed via a CCK-8 assay to evaluate the effect of PLIN3 knockdown on Huh7 and PLC/PRF/5 cells. (F, G) Wound healing and migration assays were performed to assess the migration of PLIN3-knockdown Huh7 and PLC/PRF/5 cells. (H) F-actin staining of PLIN3-knockdown Huh7 and PLC/PRF/5 cells was performed to examine cytoskeletal changes. (I) Serum and hepatic TG levels from orthotopic xenografts. (J) Representative images of liver tissues and hematoxylin-eosin-stained tumor sections from orthotopic xenografts. (K) Quantitative analysis of the orthotopic liver tumor burden. All the data are presented as the means ± standard deviation. Statistical significance is indicated as ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001.

Credit

Huiying Gu, Qiumin Wu, Haibei Zhao, JingLong Du, Zhenzhen Zhang, Juan Chen

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