IFN-γ promotes HCC metastasis via HLA-E/H2-T23 induction. (IMAGE)
Caption
(A) Quantitative RT-PCR analysis of HLA-E mRNA levels in HepG2 cells after IFN-γ stimulation. (B) Western blot analysis of HLA-E protein expression in HepG2 cells treated with IFN-γ. (C) Flow cytometric assessment of surface HLA-E expression on HepG2 cells following IFN-γ treatment. (D) Immunofluorescence staining of H2-T23 in orthotopic tumors from mice administered IFN-γ. (E) Experimental design schematic. (F) Representative gross and histological images of lung metastatic lesions. (G) Quantification of lung metastatic lesions per mouse. (H) Kaplan–Meier survival analysis.
Credit
Zhao Shu, Junfeng Guo, Lin Lv, Zhenxiang Peng, Shengtao Liao, Chuanfei Li, Zhechuan Mei
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