Validation of NDUFA11 in vivo (IMAGE)
Caption
(A) Schematic representation of the experimental paradigm. This picture was created with BioRender.com. (B) The pole-climbing test was used to examine the bradykinesia of MPTP-induced PD mice. (C) The rotarod test was used to examine the motor coordination of MPTP-induced PD mice. n = 8 per group. (D) Representative images of TH in the substantia nigra of the two groups. Scale bars: 100 μm for the original images and 50 μm for the magnified images. (E) Representative immunoblots of TH, 129P-α-synuclein, NDUFA11, and GAPDH in the control and MPTP-induced PD mice. (F–H) Quantitative analysis of the relative protein expression of (F) TH, (G) 129P-α-synuclein, and (H) NDUFA11 in the control and MPTP-induced PD mice. (I) Representative immunoblots of TH, P-α-synuclein, NDUFA11, and GAPDH in the control and A53T mice. (J–L) Quantitative analysis of the relative protein expression of (J) TH, (K) 129P-α-synuclein, and (L) NDUFA11 in the control and A53T mice. n = 3 per group. Data were shown as mean ± standard deviation. ∗P < 0.05, ∗P < 0.01, and ∗∗∗P < 0.001.
Credit
Junwei Gong, Xuesong Li, Shujuan Yang, Yajun Zhu, Xiaoguo Li, Daochen Wen, Zichao Huang, Zhao Zou, Zongduo Guo
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