Phenotypic screening of virulence-related traits in sRNA overexpression strains. (IMAGE)

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Phenotypic screening of virulence-related traits in sRNA overexpression strains.

Caption

(A, B) Biofilm formation assays comparing WTEV and the PA1014.1 overexpression strain. (C) Detection of the cupE5 gene expression in the WTEV and PA1014.1 overexpression strains by RT‒qPCR. (D, E) Biofilm formation assays comparing the WTEV and PA2734.1 overexpression strains. (F) Detection of cupE3, cupE4, and pslC gene expression in the WTEV and PA1014.1 overexpression strains by RT‒qPCR. (G) GO terms for downregulated DEGs comparing PA2736.2 overexpression strains to WTEV. (H) Swarming motility detection in the PA2736.2 overexpression strain compared to WTEV. (I) Detection of gene pilE expression in the WTEV and PA2736.2 overexpression strains by RT‒qPCR. (J) Bacterial growth competition assay between sRNA overexpression strains and E. coli. (K) Detection of T6SS-related gene expression in WTEV and PA1270.1, PA1367.1, and PA3471.1 overexpression strains by RT‒qPCR.

Credit

Tianmin Li, Beifang Lu, Fang Chen, Letong Xu, Yizhou Zhang, Fangzhou Xie, Yue Sun, Chunyan Yao, Jiadai Huang, Tianlong He, Youyue Li, Yiqing Ding, Haiyan Hu, Shaojun Tang, Xin Deng

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