The expression of Med14 targets is reduced in med14 KO embryos (IMAGE)
Caption
(A‒C) The binding of Med14 (A), Med17 (B), and Rpb1 (C) to the indicated Med14 target genes was tested by ChIP-PCR. (D) The expression of target genes was reduced in the med14 mutants. The expression of the indicated Med14 targets in the WT or med14 mutants was assessed using qPCR. Statistical significance of the difference in Med14 target expression between the WT and med14 mutants was determined using a two-tailed unpaired t-test. (E‒I) WISH was employed to validate these target genes in both the WT and mutants.
Credit
Jingwen Liu, Xuelai Liu, Feifei Li, Yujie Wang, Liping Yang, Meijia Zhang, Xinyu He, Ting Zhang, Ying Yi Zhang, Qiang Wang
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