Cell viability, mitochondrial mass, and mitochondrial DNA copy number (mtCN) in oUSCs exposed to antiretroviral drugs (IMAGE)
Caption
(A, B) Cell viability was assessed using the CCK-8 assay in USCs after 6 weeks of treatment and in HEK293 and HepG2 cells after 3 days. (C) MitoTracker Green staining of mitochondria in USCs at 6 weeks and in HEK293 and HepG2 cells at 3 days, visualized by confocal microscopy. (D–F) Quantitative analysis of mitochondrial mass based on MitoTracker Green fluorescence, expressed as an index relative to yUSCs treated with 0.1% DMSO. (G) mtDNA content in USCs was quantified by reverse transcription-PCR at 3 days and 6 weeks following drug exposure. Values were expressed as the ratio of mtDNA to nuclear DNA (NuDNA), normalized to yUSCs treated with 0.1% DMSO. (H, I) mtDNA content in HEK293 and HepG2 cells was assessed by reverse transcription-PCR at day 3, with results expressed as the mtDNA/NuDNA ratio. n = 6. Data were expressed as mean ± standard deviation. Student's t-test was used. ∗p < 0.05; ∗∗p < 0.01. ∗ versus 0.1% DMSO control in yUSCs; # versus 0.1% DMSO control in oUSCs.
Credit
Pengfei Yu, Huifen Ding, Jian-Xing Ma, Zhongping Duan, Yu Chen, Anthony Atala, Yuanyuan Zhang
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