Expression of senescence and stemness in 3D-SFM cultures (IMAGE)
Caption
(A) Senescence-associated β-galactosidase (β-gal) staining was performed in yUSCs and oUSCs after 6 weeks of culture. (B) Semi-quantitative analysis of β-gal staining intensity in (A), with yUSCs set as the baseline. (C–E) Expression of senescence-related genes (p53, p21, and Rb) in yUSCs and oUSCs at 6 weeks, assessed by quantitative PCR. (F–K) Expression of p53, p21, and Rb in HEK293 and HepG2 cells at 3 days, evaluated by quantitative PCR. (L) Telomerase activity in yUSCs and oUSCs following drug treatment for 6 weeks. HEK293 lysate was used as a representative positive control for telomerase activity. n = 6. Data were expressed as mean ± standard deviation. Student's t-test was used. ∗p < 0.05; ∗∗p < 0.01. ∗ versus 0.1% DMSO control in yUSCs; # versus 0.1% DMSO control in oUSCs.
Credit
Pengfei Yu, Huifen Ding, Jian-Xing Ma, Zhongping Duan, Yu Chen, Anthony Atala, Yuanyuan Zhang
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